Neuronal Nitric Oxide Mediates the Anti-Inflammatory Effects of Intestinal Ischemic Preconditioning
Ischemic preconditioning (IPC) can provide a defense against ischemia-reperfusion (IR)-induced acute inflammation and barrier dysfunction in many organs. Because nitric oxide (NO) has been implicated as a trigger or mediator in the IPC mechanism and because neuronal NO synthase (nNOS) is a dominant...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2019
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| Sorozat: | JOURNAL OF SURGICAL RESEARCH
244 |
| doi: | 10.1016/j.jss.2019.06.053 |
| mtmt: | 30740317 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/16846 |
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| 260 | |c 2019 | ||
| 300 | |a 241-250 | ||
| 490 | 0 | |a JOURNAL OF SURGICAL RESEARCH |v 244 | |
| 520 | 3 | |a Ischemic preconditioning (IPC) can provide a defense against ischemia-reperfusion (IR)-induced acute inflammation and barrier dysfunction in many organs. Because nitric oxide (NO) has been implicated as a trigger or mediator in the IPC mechanism and because neuronal NO synthase (nNOS) is a dominant isoform of NOS in the gastrointestinal tract, our aim was to investigate the role of nNOS in IPC-induced protection after mesenteric IR.Intestinal IR was induced in sodium pentobarbital-anesthetized dogs by clamping the superior mesenteric artery for 60 min followed by 2 h of reperfusion (IR group; n = 7). In further groups, IPC was used (three cycles of 5-min ischemia/5-min reperfusion periods) before IR in the presence or absence of selective inhibition of nNOS with 7-nitroindazole (5 mg/kg, intravenously, in a bolus 15 min before IPC, n = 6 each). Changes in mesenteric vascular resistance, intramucosal pH (pHi), and small bowel motility were monitored. Plasma nitrite/nitrate levels, intestinal NO synthase activity, leukocyte accumulation, mast cell degranulation, and histologic injury were also determined.Ischemia significantly decreased mesenteric vascular resistance and pHi, whereas IR induced a temporary bowel hypermotility and acute inflammatory reaction. IPC facilitated pHi recovery, attenuated motility dysfunction, elevated NOS-dependent NO production, and reduced leukocyte accumulation, mast cell degranulation, and mucosal injury. Pretreatment with 7-nitroindazole halted the IPC-induced increase in NO availability, pHi recovery, and the anti-inflammatory and morphologic effects.Our data demonstrate that NO generated by intestinal nNOS plays a pivotal role in IPC-linked tissue protection by inhibiting an IR-related acute inflammatory response. | |
| 700 | 0 | 1 | |a Juhász László |e aut |
| 700 | 0 | 1 | |a Galajda Péter |e aut |
| 700 | 0 | 1 | |a Bogáts Gábor |e aut |
| 700 | 0 | 1 | |a Boros Mihály |e aut |
| 700 | 0 | 1 | |a Palásthy Zsolt |e aut |
| 700 | 0 | 1 | |a Szabó Andrea |e aut |
| 700 | 0 | 1 | |a Kaszaki József |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/16846/1/VargaS-JSurgRes-2019.pdf |z Dokumentum-elérés |