Dependence of Chlorophyll Fluorescence Quenching on the Lipid-to-Protein Ratio in Reconstituted Light-Harvesting Complex II Membranes Containing Lipid Labels
The quenching of chlorophyll-a fluorescence was investigated in plant light-harvesting complex II (LHCII) embedded in reconstituted membranes containing thylakoid lipids and a lipid label. The proteoliposomes were further separated by density and the protein and lipid contents of the fractions were...
Elmentve itt :
| Szerzők: | |
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2019
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| Sorozat: | CHEMICAL PHYSICS
522 |
| Tárgyszavak: | |
| doi: | 10.1016/j.chemphys.2019.03.012 |
| mtmt: | 30629753 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/27330 |
| LEADER | 01946nab a2200253 i 4500 | ||
|---|---|---|---|
| 001 | publ27330 | ||
| 005 | 20230601154631.0 | ||
| 008 | 230601s2019 hu o 0|| Angol d | ||
| 022 | |a 0301-0104 | ||
| 024 | 7 | |a 10.1016/j.chemphys.2019.03.012 |2 doi | |
| 024 | 7 | |a 30629753 |2 mtmt | |
| 040 | |a SZTE Publicatio Repozitórium |b hun | ||
| 041 | |a Angol | ||
| 100 | 1 | |a Akhtar Parveen | |
| 245 | 1 | 0 | |a Dependence of Chlorophyll Fluorescence Quenching on the Lipid-to-Protein Ratio in Reconstituted Light-Harvesting Complex II Membranes Containing Lipid Labels |h [elektronikus dokumentum] / |c Akhtar Parveen |
| 260 | |c 2019 | ||
| 300 | |a 242-248 | ||
| 490 | 0 | |a CHEMICAL PHYSICS |v 522 | |
| 520 | 3 | |a The quenching of chlorophyll-a fluorescence was investigated in plant light-harvesting complex II (LHCII) embedded in reconstituted membranes containing thylakoid lipids and a lipid label. The proteoliposomes were further separated by density and the protein and lipid contents of the fractions were quantified spectrophotometrically, allowing tighter control over the L/P ratios in a wide range of values. Using time-resolved fluorescence, we found a strong correlation between the fluorescence quenching and the L/P ratio, in line with other studies reporting progressive quenching at low L/P ratios, presumably triggered by self-clustering in the membrane. The average fluorescence lifetimes decreased to 0.3 ns at L/P ratios below 50:1; these values are comparable to the quenching observed in plants under excess-light conditions and are accompanied by a similar far-red fluorescence signature. It is hypothesized that plants can exploit the intrinsic quenching propensity of LHCII-only membrane domains to safely store extra antenna units. | |
| 650 | 4 | |a Fizikai tudományok | |
| 700 | 0 | 1 | |a Görföl Fanni |e aut |
| 700 | 0 | 1 | |a Garab Győző |e aut |
| 700 | 0 | 1 | |a Lambrev Petar |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/27330/1/30629753.pdf |z Dokumentum-elérés |