Temoneira-1 β-lactamase is not a metalloenzyme, but its native metal ion binding sites allow for purification by immobilized metal ion affinity chromatography
β-lactamases protect bacteria from β-lactam antibiotics. Temoneira (TEM) is a class A serine β-lactamase and its coding sequence is designed into DNA vectors, such as pET-21a (+), to provide antibiotic resistance. TEM-1 β-lactamase was overexpressed efficiently from this vector upon inducing protein...
Elmentve itt :
| Szerzők: | |
|---|---|
| Dokumentumtípus: | Cikk |
| Megjelent: |
2023
|
| Sorozat: | PROTEIN EXPRESSION AND PURIFICATION
201 |
| Tárgyszavak: | |
| doi: | 10.1016/j.pep.2022.106169 |
| mtmt: | 33241868 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/28465 |