STABILON, a Novel Sequence Motif That Enhances the Expression and Accumulation of Intracellular and Secreted Proteins
The dynamic balance of transcriptional and translational regulation together with degron-controlled proteolysis shapes the ever-changing cellular proteome. While a large variety of degradation signals has been characterized, our knowledge of cis-acting protein motifs that can in vivo stabilize other...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2022
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| Sorozat: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
23 No. 15 |
| Tárgyszavak: | |
| doi: | 10.3390/ijms23158168 |
| mtmt: | 33063331 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/34615 |
| LEADER | 02342nab a2200361 i 4500 | ||
|---|---|---|---|
| 001 | publ34615 | ||
| 005 | 20240912142909.0 | ||
| 008 | 240912s2022 hu o 000 eng d | ||
| 022 | |a 1661-6596 | ||
| 024 | 7 | |a 10.3390/ijms23158168 |2 doi | |
| 024 | 7 | |a 33063331 |2 mtmt | |
| 040 | |a SZTE Publicatio Repozitórium |b hun | ||
| 041 | |a eng | ||
| 100 | 2 | |a Réthi-Nagy Zsuzsánna | |
| 245 | 1 | 0 | |a STABILON, a Novel Sequence Motif That Enhances the Expression and Accumulation of Intracellular and Secreted Proteins |h [elektronikus dokumentum] / |c Réthi-Nagy Zsuzsánna |
| 260 | |c 2022 | ||
| 300 | |a 18 | ||
| 490 | 0 | |a INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |v 23 No. 15 | |
| 520 | 3 | |a The dynamic balance of transcriptional and translational regulation together with degron-controlled proteolysis shapes the ever-changing cellular proteome. While a large variety of degradation signals has been characterized, our knowledge of cis-acting protein motifs that can in vivo stabilize otherwise short-lived proteins is very limited. We have identified and characterized a conserved 13-mer protein segment derived from the p54/Rpn10 ubiquitin receptor subunit of the Drosophila 26S proteasome, which fulfills all the characteristics of a protein stabilization motif (STABILON). Attachment of STABILON to various intracellular as well as medically relevant secreted model proteins resulted in a significant increase in their cellular or extracellular concentration in mammalian cells. We demonstrate that STABILON acts as a universal and dual function motif that, on the one hand, increases the concentration of the corresponding mRNAs and, on the other hand, prevents the degradation of short-lived fusion proteins. Therefore, STABILON may lead to a breakthrough in biomedical recombinant protein production. | |
| 650 | 4 | |a Biológiai tudományok | |
| 700 | 0 | 1 | |a Ábrahám Edit |e aut |
| 700 | 0 | 1 | |a Udvardy Katalin |e aut |
| 700 | 0 | 1 | |a Klement Éva |e aut |
| 700 | 0 | 1 | |a Darula Zsuzsanna |e aut |
| 700 | 0 | 1 | |a Pál Margit |e aut |
| 700 | 0 | 1 | |a Katona Robert L. |e aut |
| 700 | 0 | 1 | |a Tubak Vilmos |e aut |
| 700 | 0 | 1 | |a Páli Tibor |e aut |
| 700 | 0 | 1 | |a Kóta Zoltán |e aut |
| 700 | 0 | 1 | |a Sinka Rita |e aut |
| 700 | 0 | 1 | |a Udvardy Andor |e aut |
| 700 | 0 | 1 | |a Lipinszki Zoltán |e aut |
| 856 | 4 | 0 | |u http://publicatio.bibl.u-szeged.hu/34615/1/rethi_nagy_et_al_2022.pdf |z Dokumentum-elérés |