Liquid chromatographic enantioseparation of carbocyclic β-amino acids possessing limonene skeleton on macrocyclic glycopeptide-based chiral stationary phases
Polar-ionic and reversed-phase high-performance liquid chromatographic separations of limonene-based cyclic β-amino acid enantiomers were carried out by using macrocyclic glycopeptide-based chiral selectors applying Chirobiotic T, TAG and R columns. The effects of additives, concentration of the co-...
Elmentve itt :
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| Dokumentumtípus: | Cikk |
| Megjelent: |
2017
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| Sorozat: | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
145 |
| doi: | 10.1016/j.jpba.2017.06.010 |
| mtmt: | 3256483 |
| Online Access: | http://publicatio.bibl.u-szeged.hu/12656 |
| Tartalmi kivonat: | Polar-ionic and reversed-phase high-performance liquid chromatographic separations of limonene-based cyclic β-amino acid enantiomers were carried out by using macrocyclic glycopeptide-based chiral selectors applying Chirobiotic T, TAG and R columns. The effects of additives, concentration of the co- and counter-ions and the temperature in polar-ionic mobile phase systems were studied. The influence of pH, MeOH content and alcohol additives were investigated in the reversed-phase mode. The difference in the change in standard enthalpy Δ(ΔH°), entropy Δ(ΔS°), and free energy Δ(ΔG°) was calculated from the linear van't Hoff plots derived from the ln α vs 1/T curves in the temperature range 5–40 °C. Unusual temperature behavior was observed on Chirobiotic TAG for most of the analytes: decreased retention times were accompanied with increased separation factors with increasing temperature, and separation was entropically-driven. For two of the studied analytes enthalpically-driven enantioseparations were observed. The elution sequence was determined in all cases, but no general rule could be established. © 2017 Elsevier B.V. |
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| Terjedelem/Fizikai jellemzők: | 119-126 |
| ISSN: | 0731-7085 |