Studies on megacinogeny in Bacillus cereus. II. Bacillus cereus isolates characterized by prophage-controlled production of megacin A (phospholipase A)

Five out of a number of Bacillus cereus strains isolated from soil produced high titre specific bacteriocin (megacin A) in mitomycin C-induced cultures. In the course of cultivation with ethidium bromide, the strains gave off segregants not producing bacteriocin (cin-). The lysate of two wild strain...

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Elmentve itt :
Bibliográfiai részletek
Szerzők: Ivanovics G
Gaal V
Nagy Erzsébet
Prágai Béla
Simon M Jr
Dokumentumtípus: Cikk
Megjelent: 1976
Sorozat:ACTA MICROBIOLOGICA ACADEMIAE SCIENTIARUM HUNGARICAE 23 No. 3
Tárgyszavak:
mtmt:2229114
Online Access:http://publicatio.bibl.u-szeged.hu/31653
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520 3 |a Five out of a number of Bacillus cereus strains isolated from soil produced high titre specific bacteriocin (megacin A) in mitomycin C-induced cultures. In the course of cultivation with ethidium bromide, the strains gave off segregants not producing bacteriocin (cin-). The lysate of two wild strains formed plaques on the corresponding cin- bacteria. The two phages (wx23 and wx26) were identical in antigenic structure with phage wx was present in the lysate of B. cereus strain W, and converted cin- derivatives into cultures producing megacin A (phospholipase A). The phages produced plaques at 26 degrees C but not at 37 degrees C. In the lysates of the remaining three strains phages were not detected with biological and morphological methods; these cultures have been assumed to carry defective prophage genome. As the corresponding prophages are responsible for the determination of inducible phospholipase A production, phages named wx seem to form a separate group of B. cereus phages. 
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