Hplc determination of enantiomeric purity of protected amino acid derivatives used in peptide synthesis

Hplc determination of enantiomeric purity of protected amino acid derivatives used in peptide synthesis

An improved RP-HPLC method on ODS-Hypersil column with precolumn derivatization with Marfey's reagent were used to monitor racemization in N-, C- and/or side-chain protected amino acid derivatives by separation of a series of new diastereoisomeric Marfey's compounds. Chromatographic sample...

Teljes leírás

Elmentve itt :
Bibliográfiai részletek
Szerzők: Szókán Gyula
Hadfi S
Krizsán Krisztina
Liembeck A
Krecz I
Almás M
Somlai Csaba
Dokumentumtípus: Cikk
Megjelent: 1994
Sorozat:JOURNAL OF LIQUID CHROMATOGRAPHY 17 No. 13
Tárgyszavak:
Klinikai orvostan
doi:10.1080/10826079408013498

mtmt:1880327
Online Access:http://publicatio.bibl.u-szeged.hu/31995
Leíró adatok
Tartalmi kivonat:An improved RP-HPLC method on ODS-Hypersil column with precolumn derivatization with Marfey's reagent were used to monitor racemization in N-, C- and/or side-chain protected amino acid derivatives by separation of a series of new diastereoisomeric Marfey's compounds. Chromatographic samples were obtained by partial deprotection of different starting materials. In a simple two-step procedure (deprotection and derivatization) the compounds of amino acids formed stable diastereomeric derivatives having facile resolutions.
Terjedelem/Fizikai jellemzők:2759-2775
ISSN:0148-3919

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